Description
This assay employs the competitive enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to IgM. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated IgM and incubated. The competitive inhibition reaction is launched between with HRP labeled IgM and unlabeled IgM with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of IgM in the sample. The color development is stopped and the intensity of the color is measured